http://dx.doi.org/10.21276/ijlssr.2017.3.2.7

Anti-inflammatory Activity of Sting Protein from Apis mellifera

Sumesh Shah1, Amit Gupta2, 3*, Shweta P Karne1, Sanjay Kamble1, Bharat Shinde1,2, 3

1Department of Microbiology, Vidya Pratishthan’s, Arts, Science and Commerce College, Baramati, Maharashtra, India

2Department of Zoology, Vidya Pratishthan’s, Arts, Science and Commerce College, Baramati, Maharashtra, India

3Department of Immunology and Virology, Vidya Pratishthan’s School of Biotechnology (VSBT, Research Centre

affiliated to Savitribai Phule Pune University), Baramati, Maharashtra, India

*Address for Correspondence: Dr. Amit Gupta, Assistant Professor/Senior Scientist, Department of Biotechnology,

Vidya Pratishthan’s, Arts, Science and Commerce College, Baramati, Maharashtra, India

ABSTRACT- The objective of our study is to determine its anti-inflammatory potential of protein extracted from the

stings of honey bee (Apis mellifera). In this study, protein extracted from the stings of Apis mellifera using Tris HCl/ice

cold acetone and determined through Nano drop method and then determined its Da protein using SDS-PAGE. In

addition, indirect ELISA was performed using rubella vaccine as coating antigen and determined its antibody titre using

variable concentration of sting protein (15.62-250 μg) and also determined its activity on human whole blood for

determining total cellular content and proliferation against rubella vaccine antigen. The results showed that protein from

stings of Apis mellifera showed drastic declined in antibody titre at higher doses but there is slightly enhancement in

antibody titre, total cellular content and proliferations at lower concentration as compared to control and rubella vaccine

(standard).Overall, this study suggest that stings protein of Apis mellifera showed anti-inflammatory potential against

rubella vaccine antigen.

Key-words- Anti-inflammatory, Apis mellifera, Stings, Nanodrop, ELISA