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Estimation of Mass of Single Cell
Siddhesh M. Mahadik*
Department of Biotechnology, Thadomal Shahani Engineering College, Mumbai, Maharashtra, India
                                                                                                         
*Address for Correspondence: Siddhesh M. Mahadik, Department of Biotechnology, Thadomal Shahani Engineering College, Bandra (W), Mumbai- 400050, Maharashtra, India
Received: 31 December 2016/Revised: 16 January 2017/Accepted: 17 Feburary 2017

ABSTRACT- Cells are always considered as a basic unit of life in every aspect, almost whole environment consists of various types of cells. Many scientists did research on such type of organisms and they concluded almost every functioning of how micro-organisms actually work in a proper manner. Every organism has a particular morphology which can be a factor to differentiate organism based on size but in this research, it has been found that how to measure the weight of single cell whether the prokaryotic or eukaryotic cell, independent of other factors. Thus, by finding the mass and relating with its size we can even find the density of organism, may be sometimes the size cannot be a factor to detect how heavy the cell is!. Hence, using this technique one can differentiate various organisms based on their single cell weight. Many applications can be found by the respective person if someone using this technique. One of application is one can isolate aerobic bacteria also using this technique and can sub-culture it.

Key-words- Cell size, Single cell weight, Differentiate organisms, Isolation of aerobes

     
Fig.1: Structure of Eukaryotic cell Fig. 2: Structure of Prokaryotic cell     Fig. 3: Silica Gel beads 

                   Fig. 4: Principle of Mass of Single cell                  Fig. 5: Silica gel beads
                                A: Normal silica gel bead (Transparent)
                          B: Microbial growth in bead (white)


 Fig. 6: Six Microbial beads kept in sunlight denoted by 1, 2, 3, 4, 5, and 6
Observation at shadows of beads:  Bead 1: Total microbial growth throughout bead hence no light passed through it;
Bead 2-4: Light passed through the nutrient broth (orange reflection in shadow) present in the centre of beads which resembles growth of microbes on the surface only
Bead 5: shows partial growth of microbes due to depletion of nutrient broth and hence white reflection can be seen in shadow
Bead 6: No microbial growth in centre
(NOTE: For this experiment, all beads taken must be like Bead no. 1 after microbial growth hence, necessary to check under light)

CONCLUSION
The weight of single cell present in bead was 0.2 ng and hence using this technique weight of any organism can be found. If somebody wants to measure the density of particular single cell for example S. aureus whose diameter is 1µm, then the density of organism is given by (mass of single cell/ Volume of the sphere)= [m/(4/3) x π x r3]. Hence, by using this technique we can differentiate various organisms based on their single cell weight.

How to cite this article:
Mahadik SM: Estimation of Mass of Single Cell. Int. J. Life. Sci. Scienti. Res., 2017; 3(2): 887-890. DOI:10.21276/ijlssr.2017.3.2.2
Source of Financial Support: Nil, Conflict of interest: Nil


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